Listing Websites about coupon dual promoter plasmid
Filter Type:

PSF-CMV-PGK-FLUC - DUAL PROMOTER LUCIFERASE …

This plasmid contains two promoters that terminate transcription at the same poly-adenylation signal allowing the expression of two genes from one expression cassette where the second gene is the Photinus pyralis luciferase (Firefly FLuc) reporter gene. The second promoter (PGK) is approximately 10-fold weaker than the upstream CMV promoter in ...
coupon

https://www.sigmaaldrich.com/US/en/product/sigma/ogs388

Single plasmid systems for inducible dual protein

Jan 17, 2018 · The dual promoter plasmid, pNZDual (Fig. 1A), for concomitant expression of two proteins, was obtained from pNZ8148. First, the NdeI site was removed from the backbone of pNZ8148 by site directed ...
Publish Year: 2018
Author: Aleš Berlec, Katja Škrlec, Janja Kocjan, Maria Olenic, Borut Štrukelj
coupon

https://www.nature.com/articles/s41598-018-19402-1/

PSF-CMV-PGK-HYGRO - DUAL PROMOTER …

General description. This plasmid contains two promoters that terminate transcription at the same poly-adenylation signal allowing the expression of two genes from one expression cassette where the second gene is the hygromycin resistance gene (hygro) for antibiotic selection in mammalian cells. The second promoter (PGK) is approximately 10 ...
coupon

https://www.sigmaaldrich.com/US/en/product/sigma/ogs395

Plasmids 101: The Promoter Region – Let's Go!


coupon

https://blog.addgene.org/plasmids-101-the-promoter-region

Plasmids 101: Multicistronic Vectors - Addgene

Plasmids 101: Multicistronic Vectors. Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters, or the creation of bicistronic or multicistronic vectors.
coupon

https://blog.addgene.org/plasmids-101-multicistronic-vectors

Addgene: Duet011

Plasmid Duet011 from Dr. Linzhao Cheng's lab contains the insert UBC promoter - GFP - PGK promoter - Hygro and is published in Stem Cells. 2007 Mar . 25(3):779-89. This plasmid is available through Addgene.
coupon

https://www.addgene.org/17627/

Do multiple promoters with different genes within the …

Hi, if one puts three different genes under its own SV40 promoter and BGH poly A tail in one single plasmid and the last of the genes is m-Cherry tagged as a …
coupon

https://www.researchgate.net/post/Do_multiple_promoters_with_different_genes_within_the_same_plasmid_express_at_the_same_level

pDNR-Dual Sequence and Map - SnapGene

pDNR-Dual. Donor vector for transferring a cloned gene into an acceptor vector, and for tagging the gene, using Cre recombinase in the Creator™ system. Sequence Author: Clontech (TaKaRa) Open in SnapGene Try SnapGene for Free. Download Plasmid | Download SnapGene Viewer.
coupon

https://www.snapgene.com/resources/plasmid-files/?set=basic_cloning_vectors&plasmid=pDNR-Dual

Bidirectional promoter vectors - Takara Bio

Bidirectional promoter vectors. The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters ( PminCMV ). The gene (s) of interest must contain an initiation codon and a ...
coupon

https://www.takarabio.com/products/gene-function/mammalian-expression-plasmids/bidirectional-promoter-vectors

Promoters for Gene Expression | VectorBuilder

Jan 05, 2022 · MSCV. Murine embryonic stem cell virus promoter including the enhancer and promoter region of PCMV virus and 5' untranslated region of dl-587rev retrovirus. Medium-strength promoter; drives gene expression in most murine or human ES cell lines and other mammalian cell lines. Proc Natl Acad Sci U S A. 87:9202 (1990); Gene Ther. 1:136 (1994) View.
coupon

https://en.vectorbuilder.com/resources/vector-component/promoter.html

50% OFF Cmv Promoter Cell Type Verified | CouponMySun.com

PSF-CMV-CMV-SBF1-FLUC - DUAL CMV PROMOTER PLASMIDCOUPON (3 days ago) Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied.

https://couponmysun.com/cmv-promoter-cell-type

Addgene: Promoters

Definition. A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into a functional protein. Thus the promoter region controls when and where ...
coupon

https://www.addgene.org/mol-bio-reference/promoters/

In Vitro Transcription Vector (in situ hybridization

Jan 12, 2022 · This promoter is in the opposite orientation to the T7 promoter, and will generate a transcript which is the reverse-complement of that produced from the T7 promoter using the same template. BsiWI, AgeI, AscI, AvrII, XhoI, NotI, SapI: Unique restriction endonuclease sites that can be used to linearize the plasmid prior to in vitro transcription.
coupon

https://en.vectorbuilder.com/resources/vector-system/pPp_T7_SP6.html

Types of Plasmids Definition, Structure, Function, Vector

The ideal plasmid vectors have high copy numbers inside the cell. As such, it ensures high numbers of the target gene for cloning purposes. This also ensures that the gene of interest is increased during genomic division. In addition, the plasmid can have a marker gene as the visual marker to help determine whether cloning was successful.
coupon ·
dual promoter

https://www.microscopemaster.com/plasmids.html

Enhancing the Butyrylcholinesterase Activity in HEK-293

Sep 04, 2020 · However, we constructed a new dual-promoter vector to promote the plasmid’s characteristics. The designed dual-promoter vector was capable of increasing the BChE gene expression level as well as the activity of the enzyme. Composition of a plasmid, choosing the best cell line and the most suitable vector are some of the essential factors that ...
coupon

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481294/

Pluripotency Reporter Systems - BioCat

Pluripotency can be tracked in living cells using the Pluripotency Promoter Reporter Constructs. These lentivector-based constructs express copGFP or RFP under control of a stem cell-specific Oct4 or Nanog promoter. Both human and mouse Oct4 and Nanog Promoter Reporter Constructs are available. They can be applied for live cell imaging, sorting ...
coupon

https://www.biocat.com/cell-biology/stem-cell-related-products/pluripotency-reporter-systems

Bicistronic IRES vectors - Takara Bio

Details. 631970. pEF1alpha-IRES Vector. 10 ug. USD $541.00. pEF1α-IRES is a bicistronic mammalian expression vector that allows simultaneous, constitutive expression of two genes of interest from the same mRNA transcript, even after stable integration of …
coupon ·
dual promoter

https://www.takarabio.com/products/gene-function/mammalian-expression-plasmids/bicistronic-ires-vectors

Plasmids - Rockefeller University

Click the plasmid name to view or download a full-page illustration in pdf format. Illustrations show (in red) enzymes that are predicted to cut once. Other enzymes are shown that are relevant to the particular use of the plasmid. ... Original dual promoter expression vector. Unregulated T7p drives BLE: regulated GPEETp drives luciferase.
coupon

http://tryps.rockefeller.edu/trypsru2_plasmids.html

Establishment of a DGKθ Endogenous Promoter Luciferase

Sep 19, 2018 · Up to10%cash back · DGKθ protein expression levels are closely related to the development of diseases including diabetes, cancer, and neuronal disease. To investigate the transcriptional regulation of the DGKθ gene, we used CRISPR/Cas9 to generate a DGKθ endogenous promoter luciferase reporter HepG2 cell line, in which the endogenous DGKθ …
coupon

https://link.springer.com/article/10.1007%2Fs12010-018-2890-4

How does the dual promoter plasmid pnzdual work?

The dual promoter plasmid pNZDual enabled balanced expression of the two model proteins.

https://www.nature.com/articles/s41598-018-19402-1/

Filter Type: